Extended Data Fig. 7: Validation of SIC profiles by immunohistochemistry.
From: B cells are associated with survival and immunotherapy response in sarcoma
This figure refers to the NTUH cohort. a, SIC attribution as defined by gene expression using the MCP-counter Z-scores in 73 cases. b, Cell density counts showing the differences in TME composition according to SIC identification of the 73 cases (SIC A: n = 16; SIC C: n = 10; SIC E: n = 11). P values are determined by two-sided Kruskal–Wallis (KW) tests. Pairwise comparisons are derived from the Dunn test. Box plots are as in Fig. 3d. c, Representative images of CD3 (green), CD20 (pink), CD8 (brown) and CD34 (green) expression by immunohistochemistry of SIC A, C and E tumours. The same area of the tumour is represented (0.05 mm2) in each image. Similar results were observed on the other tumours from the same SICs (SIC A: n = 16; SIC C: n = 10; SIC E: n = 11).
