Extended Data Fig. 6: Single-turnover cA4 cleavage by SIRV1 gp29 and variants, and chemical rescue with imidazole.
From: An anti-CRISPR viral ring nuclease subverts type III CRISPR immunity
a, Phosphorimage of TLC visualizing cA4 cleavage by SIRV1 gp29 H47A (4 μM dimer, 50 °C) in the presence or absence of 500 mM imidazole, over time. The rate of cA4 cleavage to generate A2>P and A2-P was calculated by quantifying densiometric signals from the phosphorimage (n = 3 technical replicates). b, Plot comparing the single-turnover rates of cA4 by SIRV1 gp29, its E88A variant and its H47A variant, in the presence or absence of imidazole. Cleavage of cA4 by the H47A variant can be partially restored when the reaction is supplemented with 500 mM imidazole. Data are mean and s.d. (n = 3 technical replicates). For gel source data, see Supplementary Fig. 1.
