Extended Data Fig. 3: Excitatory latRM neurons are required for precise directional reaching.

a, Experimental scheme for injection of AAV-flex-hM4Di to the latRM of vGlut2^cre mice and representative picture of targeting specificity for behavioural experiments, counterstained for ChAT. b, Attenuation of excitatory latRM neurons does not lead to defects in open field locomotion (track length, maximal speed and length of locomotor bouts), comparing PBS and CNO trials (n = 7 mice). c, Quantitative analysis of distance to food pellet, variability and distance to mean, separately shown for PBS and CNO trial days (front camera analysis, same mice as in Fig. 2; n = 7 mice). d, Analysis of point of maximal extension for reaching trajectories using a side camera for recordings (dark coloured circles: average position of trials not missing the target; light coloured circles: same measure for missed trials; each on days with PBS or CNO injection, respectively). e, Quantitative analysis of distance to food pellet, variability and distance to mean, separately shown for PBS and CNO trial days (side camera analysis; n = 7 mice). f, Experimental design for two-choice directional reaching task with lateral and medial reaching positions (left), three examples for recorded latRM neurons (right; n = 1 neuron each), each displaying single trials aligned to behavioural phases (green: reach; yellow: grasp; magenta: retract), spikes shown as lines (top), as well as average firing rates for lateral versus medial recorded trials (bottom). g, Quantification of directionality index (sorted from medial to lateral in ascending order, n = 34 neurons) for latRM neurons recorded during the two-choice directional reaching task. Data are mean ± s.e.m. (grey shades); *P <  0.05; **P <  0.01; two-sided paired t-test.