Extended Data Fig. 5: Major synaptic targeting regions of excitatory latRM neurons.
From: A functional map for diverse forelimb actions within brainstem circuitry
a, Analysis of synaptic output derived from excitatory latRM neurons in vGlut2cre mice to the cervical spinal cord, caudal medulla and contralateral latRM. Representative pictures (left; from one of three mice used for quantification in b) and reconstructions (middle) of SynTag puncta and synaptic density (right) plots for these output structures are shown. Scale bar, 250 μm. b, Quantification of synaptic numbers along the rostro-caudal axis of the cervical spinal cord (C1, C5 and C8). The decrease in synapses between rostral and caudal cervical spinal cord segments demonstrates that spinally projecting excitatory latRM neurons terminate more strongly in rostral cervical spinal cord segments compared to caudal counterparts (n = 3 mice, data are mean ± s.e.m.). c, Summary scheme of main synaptic output areas by excitatory latRM neurons.
