Extended Data Fig. 3: STING agonists induce conditioned place preference in mice with neuropathic pain but not in naive mice.
From: STING controls nociception via type I interferon signalling in sensory neurons
a, Schematic indicating the experimental setup for the conditioned place preference (CPP) assay used to measure ongoing pain in mice. b, Schematic and timeline indicating CPP protocol for naive mice. CPP in naive mice was performed using repeated pairings (3 trials). c, Repeated pairing with i.t. morphine (2 nmol), but not DMXAA or ADU-S100 (35 nmol), induces CPP in naive mice. d, Schematic and timeline indicating CPP protocol for mice in the chemotherapy-induced peripheral neuropathy (CIPN) model of neuropathic pain. e, A single pairing with i.t. DMXAA and ADU-S100 (35 nmol) induced comparable CPP as clonidine (35 nmol), a strong analgesic when administered via i.t. injection. f, Schematic depicting patch clamp recordings in small diameter dissociated DRG neurons from STING+/+ (gWT) or STINGgt/gt (gKO) mice. g, h, STINGgt/gt mice exhibit increased excitability, as determined by number of action potentials evoked per current step (in 10 pA increments), as indicated by traces (g) and quantification (h). i, j, Nociceptors from STINGgt/gt mice also exhibit decreased rheobase at baseline, as indicated by traces (i) and quantification (j). All data are expressed as the mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001. Statistical comparisons were conducted with one-way ANOVA with Fisher’s LSD test (c, e), two-way ANOVA with Bonferroni’s post hoc test (h), or two-tailed t-test (j). See Supplementary Information for complete sample sizes, sex, and statistical information.
