Extended Data Fig. 6: SIV DNA levels in total CD4+ T cells and replication-competent reservoir size in ART-suppressed SIV-infected rhesus macaques.
From: Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo
a, Longitudinal assessment of cell-associated SIV DNA levels in total CD4+ T cells isolated from peripheral blood and lymph nodes of AZD5582-treated (n = 12, red) and control (n = 4, blue) ART-suppressed SIV-infected rhesus macaques. Grey shading represents the period of ART administration. b, Comparison of cell-associated SIV DNA levels in total CD4+ T cells isolated from the lymph nodes and peripheral blood of AZD5582-treated and control ART-suppressed SIV-infected rhesus macaques. Total CD4+ T cells were analysed from AZD5582-treated rhesus macaques 48 h after receiving 3 doses (lymph nodes and peripheral blood, n = 3) or 10 doses (lymph nodes and peripheral blood, n = 9) of AZD5582. Total CD4+ T cells were analysed from placebo-control rhesus macaques (lymph nodes and peripheral blood, n = 4) at equivalent time points. Open symbols indicate AZD5582-treated rhesus macaques with on-ART viraemia above 60 copies per ml of plasma. Statistical significance was determined using a two-sided Mann–Whitney U-test. c, Quantitative viral outgrowth assays were performed for AZD5582-treated rhesus macaques 48 h after receiving 3 doses (lymph nodes, n = 2; spleen, n = 3) or 10 doses (lymph nodes, n = 3; spleen, n = 2) of AZD5582. Quantitative viral outgrowth assays were performed from control rhesus macaques (lymph nodes and spleen, n = 4) at equivalent time points. Open symbols indicate AZD5582-treated rhesus macaques with on-ART viraemia. Statistical significance was determined with a two-sided Mann–Whitney U-test. Horizontal lines represent the median (b, c).
