Extended Data Fig. 8: Effects of the lipid membrane on βarr1 coupling to M2Rpp.
From: Structure of the M2 muscarinic receptor–β-arrestin complex in a lipid nanodisc
a, Atomistic simulations of the M2Rpp–βarr1 complex in a membrane bilayer or a small nanodisc. Time courses are provided for the calculated interdomain twist angle for each replicate in membrane bilayer (left) or a small nanodisc (right). Raw data are provided in grey and a 1-ns rolling average is provided in teal for the membrane simulations and blue for the small nanodisc simulations. Horizontal teal and blue lines correspond to an active-like and inactive-like interdomain twist angle, respectively. b, Statistical analysis of the data from Fig. 4c; data represent means of three independent experiments with error bars representing s.e.m. *P < 0.0001, one-way ANOVA; indicating significance from control within the same subset. c, Statistical analysis of data from Fig. 4d; data represent the mean and s.e.m. of three independent experiments. *P < 0.0001, one-way ANOVA; denotes significance compared to control.
