Extended Data Fig. 3: Map resolution and model validation.
From: Structure of the M2 muscarinic receptor–β-arrestin complex in a lipid nanodisc
a, ‘Gold standard’ FSC plots of the reconstruction of M2Rpp–βarr1–Fab30(scFv) (global map, black) and focused refinement reconstruction for interface M2Rpp–βarr1–Fab30(scFv) (focused map, blue). The red and brown curves represent the correlation between model and map for the global map and the focused map, respectively. Refined model validation was also performed by calculating the correlation between model and focused half map 1 (green), and the correlation between the randomly displaced model and focused half map 2 (purple). b, c, Local resolution estimation map for M2R–βarr1–Fab30(scFv) and focused map of half-M2Rpp–βarr1–Fab30(scFv). d, Overall model fit to the cryo-EM map. The 7TM portion (orange) comes from the full M2Rpp–βarr1–Fab30(scFv) map contoured at σ = 7.5, whereas the βarr1–scFv portion comes from the focused refinement of Interface M2Rpp–βarr1–Fab30(scFv) contoured at σ = 5.2.
