Extended Data Fig. 10: Cell distribution in D-, M- and R-type cavities before and after Cy/GCSF treatment.
From: Live-animal imaging of native haematopoietic stem and progenitor cells
n = 4 mice per group. Graphs show the fractions of MDS or MFG cells distributed in D-, M- and R-type cavities at the steady state and after Cy/GCSF treatment. The fraction is calculated by the total cells found in each cavity type divided by the total cells found in the calvaria of that mouse. a, The fractions of MFG cells increased in M-type cavities but decreased in D-type cavities after Cy/GCSF treatment. Mean ± s.d. Non-treated groups: 24.5 ± 12.8, 54.3 ± 12.6 and 21.3 ± 15.6 in D-, M- and R-type cavities, respectively. Treated groups: 0.5 ± 1.0, 96.0 ± 4.7 and 3.5 ± 4.4 in D-, M- and R-type cavities, respectively. **P = 0.0096; *** P = 0.0008. b, The fractions of cells decreased in D-type cavities but remained the same in M- and R-type cavities. Mean ± s.d. Non-treated groups: 20.5 ± 5.6, 66.5 ± 2.4 and 13.3 ± 3.6 in D-, M- and R-type cavities, respectively. Treated groups: 6.8 ± 2.5, 75.0 ± 9.6 and 18.8 ± 8.9 in D-, M- and R-type cavities, respectively. **P = 0.004; unpaired, two-tailed t-test.
