Extended Data Fig. 3: Generation of the MFG (Mds1^GFP/+ Flt3^Cre) mice results in restriction of GFP expression to LT-HSCs.

a, Schematic of genetic strategy to restrict GFP expression to LT-HSC compartment. b, Analysis of bone marrow from Flt3^CreR26LSL-Tom mice shows Flt3^Cre-driven activity in compartments downstream of LT-HSCs. n = 4 mice; mean ± s.d. c, Further characterization of the CKIT⁺SCA1⁻GFP⁺ cells from MFG mice. CD41⁺CD150⁺ cells represent  megakaryocyte progenitors. The experiment was performed twice with similar results. d, Flow characterization of MFG cell in marrow isolated from multiple bones. The experiment was performed three times with similar results. e, MFG-HSCs are predominantly found within the CD34⁻Flt3⁻CD150⁺ bone marrow fraction. The experiment was performed twice with similar results. f, MFG-HSCs are predominantly found within the SCA1^highEPCR⁺ bone marrow fraction. The experiment was performed once. g, Cell cycle analysis of SLAM cells that are either GFP⁺ or GFP⁻ in MFG mice. Pooled data from three mice.

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