Extended Data Fig. 2: Differential expression of enteric-neuron-specific genes in myenteric neurons from the small intestine and colon of SPF and germ-free mice.
From: Neuronal programming by microbiota regulates intestinal physiology
a, b, Representative images of myenteric ganglia (outlined by dotted line) from small intestine (left) and colon (right) of SPF (a) and germ-free (b) mice hybridized with the indicated fluorescence RNAscope probes and counterstained for the pan-neuronal marker HuC/D. Ret (positive control for RNAscope detection) is expressed in neurons of myenteric ganglia of both the small intestine and the colon. Pou3f3, Pde1c, Pantr2, Ano5, Unc5d and Col25a1 are expressed at higher levels in colonic versus small intestine neurons in both SPF (a) and germ-free (b) mice. Data represent three independent experiments. Transcripts per kilobase million (TPM) values (mean ± s.d.) for each transcript in small intestine and colon neurons from SPF (a) and germ-free (b) mice. n = 8 SPF and 3 germ-free mice. Scale bars, 30 μm.
