Extended Data Fig. 3: Additional single-cell transcriptomic characterization.

a, UMAP⁴² representation of 20,257 Pogona telencephalic cells, colour-coded by cluster. EG, ependymoglial cells; ExcNeur, excitatory neurons; InhNeur, inhibitory neurons; MG, microglia; mur, mural cells; NPC, neural progenitor cells; olig, oligodendrocytes; OPC, oligodendrocyte progenitor cells; prol, proliferating cells; RBC, red blood cells. b, Dot plot showing the expression of canonical cell markers (rows) across telencephalic cell clusters (columns). The size of the dot corresponds to the percentage of cells in a cluster in which the gene has been detected, and the colour represents the expression level. c, UMAP representation of 9,777 lizard telencephalic neurons, colour-coded by cluster. d, e, UMAP representations of glutamatergic (slc17a7) and GABAergic (slc32a1) neurons in the telencephalon dataset. f, Double colorimetric in situ hybridization in a frontal section through the anterior Pogona forebrain. Scale bar, 1 mm. slc32a1 (blue) labels GABAergic neurons in the subpallium and scattered GABAergic neurons that have migrated from subpallium to pallium. slc17a6 (orange) labels glutamatergic neurons in the pallial region. g, Ordered matrix of pairwise Pearson correlations between the expression of 143 ion-channel and neurotransmitter-receptor genes detected in this glutamatergic pallial dataset from Pogona (see Extended Data Fig. 5). The dendrogram (top) is based on correlation coefficients and Ward.D2 linkage; red indicates a gene module with enriched expression in the amDVR. h, Average expression, in the 29 glutamatergic Pogona clusters, of the 143 genes in g (and Extended Data Fig. 5). Genes with enriched expression in the amDVR are listed on the right, with relevant neurotransmitter receptor genes in bold. i, UMAP representation of 4,054 lizard pallial glutamatergic neurons, colour-coded by cluster (same as in Fig. 3a). j, Dot plot showing the expression of specific cluster markers (rows) in the 29 pallial glutamatergic clusters (columns). The size of the dot corresponds to the percentage of cells in a cluster in which the gene has been detected, and the colour represents the expression level.