Extended Data Fig. 5: Analysis of Nfe2l2–Mafg signature by Ribotag and scRNA-seq. | Nature

Extended Data Fig. 5: Analysis of Nfe2l2–Mafg signature by Ribotag and scRNA-seq.

From: MAFG-driven astrocytes promote CNS inflammation

Extended Data Fig. 5: Analysis of Nfe2l2–Mafg signature by Ribotag and scRNA-seq.The alternative text for this image may have been generated using AI.

a, EAE in mice used in RibotagGfap studies. n = 3 mice per time point. b, RINs for Ribotag preparation. IP, immunoprecipitated HA-tagged ribosomes. mRNA direct, enrichment of polyadenylated mRNA using mRNA direct kit (Thermo Fisher, #61011). n = 4 biologically independent samples per condition. c, K-means clustering of RibotagGfap RNA-seq data for five CNS regions. d, ENRICHR analysis of upregulated genes in EAE (top). Analysis of gene expression associated with the altered glutathione metabolism KEGG pathway by CNS region (bottom). Number of independent mouse samples studied: n = 7 cortex, n = 8 spinal cord, n = 7 parenchyma, n = 9 cerebellum, n = 8 cranial nerves. e, Gene expression scatterplots of genes of interest in B6 EAE scRNA-seq studies. n = 24,275 cells. Data shown as mean ± s.e.m.

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