Extended Data Fig. 4: VAT T_reg cell extrinsic function of sex hormones.

a, Schematic shows the strategy used to make bone marrow chimeric mice using wild-type and Ar^−/− recipients. b, Proportions of T_reg cells from the VAT of irradiated wild-type (n = 5) and Ar^−/− (n = 6) mice that received wild-type bone marrow. Quantification on the right. c, Expression of indicated cell surface markers on VAT T_reg cells from wild-type and Ar^−/− mice that were reconstituted with wild-type bone marrow (from b). d, Flow cytometry plots (left) show expression of FOXP3 and ST2 in VAT CD4⁺ T cells from male Ar^fl/flFoxp3^cre (n = 6) and Foxp3^cre (n = 4) control mice. Quantification on the right. e, Expression of CCR2 and KLRG1 in VAT T_reg cells from Ar^fl/flFoxp3^cre and control mice (from d). f, Percentages of wild-type and Era^−/− T_reg cells in the VAT of female bone marrow chimeric mice. Irradiated wild-type female Ly5.1 recipient mice were reconstituted with a mixture of female Ly5.2 wild-type (n = 4) and female Era^−/− (n = 5) bone marrow cells. g, h, Percentages of splenic T_reg cells in oestrogen-treated (n = 12) and untreated (n = 6) male wild-type mice (g) and in testosterone-treated (n = 9) and untreated (n = 5) female wild-type mice (h). i, Expression of FOXP3 and CD25 in VAT CD4⁺ T cells isolated from oestrogen-treated or untreated male wild-type mice. j, Flow cytometry histograms show expression of KLRG1 and ST2 in VAT T_reg cells from oestrogen-treated or untreated male wild-type mice. k, Expression of FOXP3 and CD25 in VAT CD4+ T cells isolated from testosterone-treated or untreated female wild-type mice. l, Expression of KLRG1 and ST2 in VAT T_reg cells from testosterone-treated or untreated female wild-type mice. Two-tailed unpaired t-test. Data are mean ± s.d. Data are pooled or representative of two independent experiments.

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