Extended Data Fig. 2: Data processing and structure determination of the substrate-free ALG6–6AG9-Fab complex in lipid nanodiscs.
From: Structure and mechanism of the ER-based glucosyltransferase ALG6
a, Overview of the EM data processing and structure determination pipeline using RELION 3.046. b, Representative cryo-EM micrograph. c, Representative 2D classes. d, A representative 2D class with 6AG9-Fab and the nanodisc belt highlighted. e, Spatial distribution of particles in the final iteration of 3D refinement. f, Refined and B-factor-sharpened EM map, coloured by local resolution estimation as calculated in ResMap52. g, Resolution estimation of the final map via Fourier shell correlation (FSC), as calculated in RELION 3.0. h, The FSC between model and map, as calculated in PHENIX mtriage51.
