Extended Data Fig. 2: Multi-photon ablation protocol.

a, Example field of view immediately before (left) and after (right) ablation. Orange arrow denotes target neuron. b, Ablation protocol. Power (orange, top trace) during ablation epochs (100 ms; increased power, orange, top) gradually increased, and the PMT shutter was closed (black bars). During the intervening evaluation epochs, power was lower and constant (orange, top), and the PMT shutter was open. Ablation was terminated when GCaMP fluorescence at the target neuron (green) jumped (orange arrow). c, Success of ablation as a function of neuron depth for all experiments included in this dataset. Individual points give mean success rate for given depth bin; bin size, 25 μm. d, Depth dependence of total energy deposition for successful ablations (successfully ablated cells only: n = 293 cells across 22 sessions, 14 mice; ablations from j250220 and j257218, along with 7 additional ablations from other mice were excluded owing to incomplete logging). Grey dots denote individual ablations. Black dots, means across 25-μm bins. e, As in d, but for peak power needed for ablation (n = 293 cells across 22 sessions, 14 mice).