Extended Data Fig. 6: GSDME overexpression in B16 cells reduces tumour growth and increases immune responses within tumours.
From: Gasdermin E suppresses tumour growth by activating anti-tumour immunity
a, Tumour growth in C57BL/6 mice that were implanted subcutaneously with B16 cells stably transduced with empty vector (n = 6 mice per group) or expressing mGSDME (n = 8 mice per group) or inactive F2A GSDME (n = 5 mice per group). b, Numbers of CD8+ (left) and NK (middle) TILs and tumour-associated macrophages (right) in tumours, normalized to tumour weight. c, Percentage of CD8+ TILs expressing GzmB or PFN (left), and IFNγ or TNF after PMA and ionomycin stimulation (right). d, Percentage of NK cells expressing GzmB or PFN. The area under the curve of tumour growth curves in a was compared by one-way ANOVA with Holm–Sidak correction for type I errors. Comparisons in b–d were calculated by one-way ANOVA using the Holm–Sidak method for multiple comparisons. Data are mean + s.e.m. All data are representative of at least two independent experiments.
