Extended Data Fig. 10: PFN plus GzmB induce pyroptosis in SH-SY5Y cells.
From: Gasdermin E suppresses tumour growth by activating anti-tumour immunity
a–c, SH-SY5Y cells treated with PFN with or without GzmB or medium for 2 h were analysed by microscopy (a), immunoblot of cell lysates probed for GSDME and actin (c) or LDH release (b). In b, c, treatment was in the presence of 30 μM of caspase inhibitors as indicated. d, Effects of GSDME knockout on pyroptosis induced by PFN with or without GzmB, assessed after 1 h of treatment by LDH release. Differences among multiple groups in b, d were analysed by one-way ANOVA using the Holm–Sidak method for multiple comparisons. Data are mean ± s.d. of biological triplicate wells. ***P < 0.0001. Data are representative of two independent experiments.
