Extended Data Fig. 4: Gsdme knockout in EMT6 and CT26 cells increases tumour growth and reduces immune responses within tumours.
From: Gasdermin E suppresses tumour growth by activating anti-tumour immunity
a–j, Comparison of control and Gsdme−/− orthotopic EMT6 tumours (control n = 6, Gsdme−/−n = 7) (a–e) and subcutaneous CT26 tumours (control n = 6, Gsdme−/−n = 5) (f–j) in BALB/c mice. The effects of knockout are demonstrated in Extended Data Fig. 1. Shown are tumour growth (a, f); numbers of CD8+ TILs (left), NK cells (middle) and tumour-associated macrophages (right), normalized to tumour weight (b); representative flow plots (left) and mean percentage of CD8+ TILs expressing GzmB or PFN (right) (c, g); mean percentages of NK cells expressing GzmB or PFN (d, h); and representative flow plots (left) and mean percentage of CD8+ TILs (right, e, i) and CD4+ TILs (j) producing IFNγ or TNF after PMA and ionomycin stimulation. The area under the curve in a, f and differences between two groups in b–e, g–j were compared by two-tailed Student’s t-test. Data are mean + s.e.m. Data are representative of at least two independent experiments.
