Extended Data Fig. 5: Effects of GSDME expression on tumour growth and immune responses within 4T1E tumours.
From: Gasdermin E suppresses tumour growth by activating anti-tumour immunity
a, 4T1E cells stably expressing wild-type mGSDME (n = 6 mice per group), the inactive F2A mutant of mGSDME (n = 6 mice per group) or an empty vector (n = 7 mice per group), implanted in mammary fat pads, were analysed for tumour-infiltrating immune-cell numbers. b–e, 4T1E cells, stably expressing eGFP and then stably transduced to express mGSDME or empty vector (b), were compared for raptinal-induced SYTOX green uptake in vitro (c), tumour growth after orthotopic implantation (n = 7 mice per group) (d), and percentage of CD8+ TILs expressing GzmB or PFN (e). Comparisons in a were calculated by one-way ANOVA using the Holm–Sidak method for multiple comparisons; comparisons in e were calculated by two-tailed Student’s t-test; comparisons in c, d were calculated by comparing the difference between the areas under the curve by two-tailed Student’s t-test. Data shown are mean + s.e.m. ***P < 0.0001. All data are representative of two independent experiments.
