Extended Data Fig. 10: Hodor is an insect-specific gene, and is essential in A. gambiae.
From: An intestinal zinc sensor regulates food intake and developmental growth
a, Nucleotide-level maximum likelihood phylogeny of the hodor gene family, highlighting successive duplication events at the base of the Schizophora (orange and red nodes, see Methods for details of phylogenetic reconstruction, and Supplementary Information for a complete gene family tree). Bootstrap support is indicated along individual branches as a percentage of 1,000 rapid bootstraps. b, gRNA target site within exon 2 of the A. gambiae one-to-many orthologue AGAP009616 of fly hodor-like genes, the diagnostic primers used for genotyping and the three frameshift mutants recovered. PAM, protospacer adjacent motif. c, Strategy for the recovery of AGAP009616 mutants. d, Genotyping the progeny of crosses between verified heterozygote males and females revealed that AGAP009616 homozygous mutant adults are inviable. See Methods for details.
