Extended Data Fig. 3: Conserved metabolite release during apoptosis.
From: Metabolites released from apoptotic cells act as tissue messengers
a, Mass spectrometry was used to measure the concentration of the five metabolites that were released across all conditions and platforms tested, in live or apoptotic supernatants per million Jurkat T cells or isolated primary thymocytes (back-calculated from total cells used in experimental set-up) (n = 3). Metabolites are grouped by metabolic pathways. Data are mean ± s.d. *P = 0.014, **P = 0.0014, ***P = 0.0002, ****P < 0.0001, unpaired two-tailed Student’s t-test. b, The concentration of ATP released in the supernatant across the different apoptotic Jurkat cells was determined by luciferase assay (n = 4). Data are mean ± s.e.m. ****P < 0.0001, ordinary one-way ANOVA with Turkey’s multiple comparison test. c, Table outlining the different cell types, apoptotic stimuli, techniques and metabolites screened for untargeted (more than 3,000 features or compounds) and targeted (116 metabolites) metabolomics, including ATP, spermidine, glycerol-3-phosphate and creatine.
