Extended Data Fig. 4: PANX1 activation and inhibition during cell death.
From: Metabolites released from apoptotic cells act as tissue messengers
a, Top, representative histograms of TO-PRO-3 dye uptake in thymocytes across the different conditions. Bottom, PANX1 activation in live and apoptotic thymocytes from wild-type (Panx1+/+) and PANX1-knockout (Panx1−/−) mice as assessed via flow cytometry by measuring the mean fluorescent intensity of TO-PRO-3 dye uptake (n = 3 biological replicates). Data are mean ± s.e.m. ****P < 0.0001, ordinary one-way ANOVA with Turkey’s multiple comparison test. b, Top, representative histograms of TO-PRO-3 dye uptake in Jurkat cells, across the different conditions described. Bottom, PANX1 activation as assessed by flow cytometry of the uptake of TO-PRO-3 dye in apoptotic wild-type Jurkat cells, Jurkat cells expressing mutant PANX1-DN, and Jurkat cells treated with PANX1 inhibitor spironolactone (50 μM) or trovafloxacin (25 μM) (n = 4 biological replicates). Data are mean ± s.e.m. ****P < 0.0001, ordinary one-way ANOVA with Turkey’s multiple comparison test.
