Extended Data Fig. 6: Structural tuning at maternal early domains during preimplantation.
From: Parental-to-embryo switch of chromosome organization in early embryogenesis
a, Snapshots of HiC matrices and H3K27me3 ChIP–seq signal, showing the parental differences between the 2-cell and 64-cell stages for maternal (red) and paternal (blue) genomes at chromosome 2 (9–13.5 Mb) containing Sfmbt2. b, As in a, for chromosome 3 (40–43 Mb) containing Jade1. c, Quantification of contacts within the region presented in Fig. 3b. d, Snapshot of HiC matrices and H3K27me3 ChIP–seq signal, showing the parental differences between the 2-cell and 64-cell stages for maternal (red) and paternal (blue) genomes at chromosome 9 (60–62.5 Mb) containing Tle3. e, Gene-expression dynamic for Tle3 for maternal (red) and paternal (blue) alleles. f, Quantification of contacts within the region shown in d. g, Snapshots of HiC matrices and H3K27me3 ChIP–seq signal, showing the parental differences between the 2-cell and 64-cell stages for maternal (red) and paternal (blue) genomes at chromosome 13 (96–100 Mb) containing Enc1. h, As in g, for chromosome 14 (115–122 Mb) containing Mbnl2. i, Relative gene expression for Xist (in red) or Jpx (in yellow) in mouse embryonic fibroblasts derived from embryos issued from crossing ∆Jpx/wild-type female mice with wild-type/Y or ∆Jpx/Y male mice. The three genotypes analysed are indicated, as well as the number of independently derived mouse embryonic fibroblast cultures from independent single embryos (n = 4, 6 and 6 for wild-type/wild-type, wild-type/∆Jpx and ∆Jpx/∆Jpx genotypes, respectively). Bar plot represents the mean of each independent expression value (for each embryo), error bars represent the s.d. and each dot represents an individual embryo value. j, Pie chart distribution of the genotypes obtained after mating ∆Jpx/wild-type female mice with ∆Jpx/Y male mice. n = 104 pups.
