Extended Data Fig. 3: DREADD-mediated excitation of Q neurons.
From: A discrete neuronal circuit induces a hibernation-like state in rodents
a, Qrfp mRNA is expressed in mCherry-positive neurons in Q-hM3D mice. Dual-colour in situ hybridization for Qrfp and mCherry mRNA in brain slices prepared from Q-hM3D mice. We confirmed that CNO administration induced QIH, and subjected the mice to histological analysis. All mCherry-positive neurons were positive for Qrfp expression. Scale bars, 100 μm (left); 10 μm (middle, right). b, Representative trace of current-clamp recording from mCherry-positive Q neurons in a slice prepared from Q-hM3D mice. We performed the experiments nine times and obtained the same results. c, Comparison of spike frequency at baseline and after treatment with CNO (n = 9). d, Estimated distribution of spike frequency in baseline and CNO-treated slices. e, The estimated difference in spike frequency between CNO-treated and baseline slices was [1.44, 2.80] Hz. Because the 89% HPDI of the estimated difference is positive, the spike frequency in CNO-treated slices may be larger than baseline by more than 89%.
