Extended Data Fig. 3: EMSAs of SOX2 and SOX11 in complex with DNA or nucleosomes.
From: Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function
Related to Figs. 1–3. EMSAs reveal formation of SOX-factor complexes with DNA (left) or nucleosomes (right). DNA or nucleosome concentration is 1.1 nM. a, EMSA of DNA-1–SOX2 and nucleosome-1–SOX2 complexes. b, EMSA of DNA-1–SOX11 and nucleosome-1–SOX11 complexes. c, EMSA of DNA-1a–SOX11 and nucleosome-1a–SOX11 complexes. d, EMSA of DNA–Widom+1 and nucleosome–Widom+1–SOX11 complexes. e, EMSA of DNA Widom 601–SOX11 and nucleosome Widom 601–SOX11 complexes. Relevant bands are labelled. All experiments were repeated at least twice with similar results. For gel source data, see Supplementary Fig. 1.
