Extended Data Fig. 9: AAV8-tdTomato-Cre expression completely abolishes detection of HTT protein six weeks after injection.
From: Injured adult neurons regress to an embryonic transcriptional growth state
AAV8-dependent Cre delivery to Httflox/flox mice was used to conditionally knock out HTT expression in corticospinal neurons six weeks before injury. a–c, Low magnification of right motor cortex six weeks after AAV8-tdTomato-Cre injection indicates successful tdTomato (red) expression in motor cortex and reduction in HTT protein (black or blue) in the infected area. d, e, High-magnification images showing cortical neurons with baseline HTT protein expression outside of infected areas (d) and after HTT deletion in cortical neurons that are transfected with AAV8-tdTomato-Cre (e). f, AAV8-tdTomato-Cre-infected cortical neurons co-express tdTomato (red) and Cre (green). n = 3 biological replicates, with comparable outcomes.
