Extended Data Fig. 1: TRAP of regenerating corticospinal neurons.
From: Injured adult neurons regress to an embryonic transcriptional growth state
a, b, Coronal cortical section of transgenic BAC–TRAP mice expressing eGFP–L10A under the Glt25d2 promoter primarily in neurons (NeuN) of cortical layer 5b. b, Confocal z-stack projection of 15-μm thickness showing colocalization of eGFP–L10A and NeuN within pyramidal neurons. c, d, Retrogradely labelled CST neurons (CTB) projecting to the cervical spinal cord (C4–C8) grey matter colabel with eGFP–L10A, indicating that about 50% of TRAPed mRNA molecules are derived from corticospinal neurons. d, Confocal z-stack projection of 10-μm thickness showing colocalization of eGFP–L10A and CTB within pyramidal neurons. n = 3 biological replicates, which showed comparable results. e, Experimental timeline for purification of TRAPed mRNA molecules exclusively from cortical-layer-5 neurons.
