Extended Data Fig. 5: Verification of RNA-seq data via immunolabelling for ATG7, SOX9 and PAX2.
From: Injured adult neurons regress to an embryonic transcriptional growth state
Immunohistological staining of selected proteins in layer 5b of the motor cortex of Glt25d2-eGFP-L10a transgenic mice. a, b, eGFP–L10A staining is shown in green and SOX9 staining is shown in red in intact mice (a) or in regenerating CST 10 days after injury with E12 NPC graft (b). c, Sox9 mRNA expression after TRAP and RNA-seq. Data are normalized to those from intact mice. ****P = 3.2 × 10−7, two-tailed t-test. d, e, eGFP–L10A staining is shown in green and PAX2 staining is shown in red in intact mice (d) or in regenerating CST 10 days after injury with E12 NPC graft (e). f, Pax2 mRNA expression after TRAP and RNA-seq. Data are normalized to those from intact mice. **P = 0.0057, two-tailed t-test. g, h, eGFP–L10A staining is shown in green and ATG7 staining is shown in red in intact mice (g) or in regenerating CST 10 days after injury with E12 NPC graft (h). i, Atg7 mRNA expression after TRAP and RNA-seq. Data are normalized to those from intact mice. ****P = 3.0 × 10−6, two-tailed t-test. n = 3 biological replicates per condition. Data are mean ± s.d.
