Extended Data Fig. 6: NPY signalling in the IGL–SCN circuit controls nonphotic entrainment.
From: Retinal innervation tunes circuits that drive nonphotic entrainment to food
a–d, Representative actograms obtained from NPY-knockout (KO) (Npycre/cre) mice exposed to TRF are shown (a). The locomotor activity 9 h before food access (b) and the food-anticipatory activity (c) were measured for control and NPY-knockout mice. Data are mean ± s.e.m. (n = 7 control mice, 11 NPY-knockout mice), two-tailed Student’s t-test. Additionally, a score analysis was performed for all actograms obtained (d). Data are mean ± s.e.m. (n = 7 control mice, 11 NPY-knockout mice), Student’s t non-parametric (Mann–Whitney) test, two-tailed. In addition, a second mouse line (Npytm1Rpa) was used to evaluate the effects of NPY ablation. Results obtained from both NPY-knockout mouse lines were indistinguishable (data not shown). e, The daily total amount of food consumed (during ad libitum access to food and TRF) was measured in control, NPY-knockout (Npycre/cre), and Npycre/+ mice bilaterally injected in the IGL with a control AAV (AAV5/Syn-DIO-hChR2(H134R)-EGFP-WPRE-HGHpA) or AA encoding TenT (pAAV5/CMV-DIO-eGFP-2A-TeNT). Under free-running conditions (constant darkness and ad libitum access to food), the amount of food consumed by control and NPY-knockout mice (P = 0.0267), as well as control and Npycre/+ mice (P = 0.008), was significantly different. Data are mean ± s.e.m.(n = 5 mice for each condition), two-way ANOVA, followed by Sidak’s multiple comparisons test. f, g, Representative actograms obtained from Npycre/+ mice exposed to TRF and injected with control AAV (f) or AAV encoding TenT (g) are shown.
