Extended Data Fig. 8: Anti-PfGARP antibodies disrupt the integrity of the food vacuole.
From: Anti-PfGARP activates programmed cell death of parasites and reduces severe malaria
3D7 parasites were synchronized to the ring stage and plated at 5% parasitaemia in the presence of pre-immune (a) or anti-rPfGARP-A (b) mouse serum at a 1:10 dilution. Parasites were cultured for 24 h and processed for transmission electron microscopy. Data are representative of all trophozoites observed in three biologically independent experiments (a, b). c, Ring-stage 3D7 parasites were treated with pre-immune or anti-PfGARP serum (1:10) or 1 μM chloroquine for 24 h, followed by staining with DAPI and Fluo-4 AM. Data are representative of two biologically independent experiments.
