Extended Data Fig. 10: The PEXEL motif is processed and cleaved in mature PfGARP.
From: Anti-PfGARP activates programmed cell death of parasites and reduces severe malaria
a, Schematic depicting the binding sites for the peptide-specific antibodies. b–e, Immunoblot of rPfGARP-A (lane 1), recombinant full-length PfGARP (lane 2) and an extract of trophozoite-infected RBCs (lane 3) probed with antibodies raised against amino acids 504–522 of PfGARP (b) or pre-immune serum (c), or with antibodies raised against amino acids 31–48 of PfGARP (d) or pre-immune serum (e). Only antibodies raised against amino acids 504–522 recognized native PfGARP in trophozoite-infected RBCs, whereas antibodies raised against amino acids 31–48 only recognized the full-length recombinant PfGARP, confirming that the PEXEL motif is cleaved during the processing of native PfGARP. Pxl, PEXEL motif; SP, signal peptide. Data are representative of three biologically independent experiments.
