Extended Data Fig. 2: Characterization of recombinant monoclonal anti-PfGARP.

a, The kinetics of binding between the recombinant monoclonal antibody mAb7899 and PfGARP-A were measured at two antibody concentrations. For each concentration, two biologically independent replicates were performed. The experiment was performed twice and the data were pooled. The dashed lines represent the 95% CI. Error bars represent s.d. The formula is a linear regression. C, concentration of biotinylated recombinant mAb7899; MFI, median fluorescence intensity; V, initial velocity of binding. b, Epitope mapping of recombinant mAb7899. We printed a custom 15-mer-peptide microarray containing 264 different peptides that spanned the PfGARP-A sequence (amino acids 410–673). The peptides overlapped by a single amino acid and were printed in duplicate, framed by haemagglutinin (HA) control peptides. The array was probed with recombinant mAb7899 (red) and anti-HA (green) and imaged on an LI-COR Odyssey. c, RBCs infected with ring-stage 3D7 parasites were cultured in the presence of medium alone, recombinant anti-PfGARP monoclonal antibody or recombinant anti-fluorescein. Parasites were cultured for 48 h at 37 °C and the levels of lactate were measured in the culture supernatant. Data are mean ± s.e.m. of four biologically independent replicates. The P value was calculated by non-parametric two-sided Mann–Whitney U-test. d, RBCs infected with ring-stage 3D7, D10 or Dd2 parasites were cultured in the presence of medium alone or recombinant anti-PfGARP Fab antibody (1 mg ml⁻¹) for 48 h at 37 °C, and ring-stage or early-trophozoite-stage parasites were counted by microscopy. Data are mean ± s.e.m. of three biologically independent replicates. P values were calculated by non-parametric two-sided Mann–Whitney U-test. Data are representative of two independent experiments.