Extended Data Fig. 5: Inducible knockout of Meis1 and Hoxb13 promotes sarcomere disassembly and cardiomyocyte division.
From: A calcineurin–Hoxb13 axis regulates growth mode of mammalian cardiomyocytes
a, Representative images of Masson’s trichrome staining of DiKO hearts four weeks after deletion. b, c, Disassembled sarcomere cardiomyocytes in DiKO hearts one week after deletion, demonstrated by cardiac troponin T (red) (b) or sarcomeric α-actinin (red) (c). d, Representative images of immunostaining for TUNEL (green) and cardiac troponin T (red) in DiKO hearts one week and two months after deletion, as shown in Fig. 2e. e, f, Representative images of immunostaining for PH3 (green), cardiac troponin T (red) and nucleus (blue), showing additional examples of proliferating cardiomyocytes in DiKO hearts at (e) one week and (f) two months after gene deletion. g, Representative images of immunostaining for aurora B kinase (green), cardiac troponin T (red) and nucleus (blue) (left) and the percentage (right) of proliferating cardiomyocytes (arrow) one week after gene deletion. h, Additional examples of cytokinetic cardiomyocytes in DiKO hearts one week after gene deletion. Images in a–c are representative of three independently performed experiments with similar results. Scale bars, 10 μm (b, d–h), 20 μm (c), 1 mm (a).
