Extended Data Fig. 6: Cis-looping and alternative model for pericentromeric chromosome conformation.
a, Pile-ups (bin size 1 kb) of cis contacts located in the 100 kb around all 16 centromeres for wild-type and chl4Δ cells in the absence or presence of spindle tension. b, Pile-ups of cis contacts in the 100 kb around centromeres (left); ratio of expected/observed signals (second panel); pericentromere pile-up (third panel, 10 kb surrounding centromeres); and its ratio of expected/observed signals (right), for wild-type cells in the absence of spindle tension (n = 16). c, Model proposed previously19 showing the formation of intramolecular loops by centromere-flanking pericentromeric chromatin, with cohesin bridging the two sides of the pericentromere flanking the centromere; meanwhile chromosome arms are paired intermolecularly between sister chromatids, resulting in a cruciform chromosome conformation.
