Extended Data Fig. 1: Tension-dependent cohesin removal at metaphase is restricted to the pericentromere and occurs independently of Wpl1/Rad61.

a, Scc1–6HA-calibrated ChIP-seq profiles for the pericentromeric region of chromosome IV are shown for rad61Δ cells arrested in metaphase, in the absence and presence of spindle tension (n = 1). b, Mean calibrated ChIP reads (solid lines), standard errors (dark shading) and 95% confidence intervals (light shading) at a pericentromere-proximal cohesin site on each chromosome arm (n = 32 sites), for wild-type and chl4Δ cells, in either the presence or the absence of tension.