Extended Data Fig. 4: Shugoshin and condensin localize to pericentromere borders.

a, Representative cohesin (Scc1), shugoshin (Sgo1) and condensin (Brn1) enrichment in metaphase-arrested cells in the presence of nocodazole in the pericentromeric region of chromosome IV (n = 2; immunoprecipitation was performed using proteins tagged with different epitopes in the two biological replicates). b, Plots show mean calibrated ChIP reads (solid lines), standard errors (dark shading) and 95% confidence intervals (light shading) at all 32 borders and 16 centromeres. For comparison, similar plots for the next convergent gene site on each chromosome arm are shown. c, d, Condensin associates with pericentromere borders in a Sgo1-dependent manner in cells arrested in metaphase in the absence of tension. c, These ChIP-seq data have been published previously¹⁴, and show the median condensin (Brn1) signal across a 50-kb region surrounding all 16 centromeres. d, The mean Brn1 signal centred around all 32 borders (left) or 16 centromeres (right). Sgo1 is removed from the borders, but not from core centromeres, in response to spindle tension (solid lines show medians; dark shading shows standard errors; light shading shows 95% confidence intervals). e, Median Sgo1 enrichment by ChIP-seq, plotted over a 50-kb region surrounding all 16 centromeres in metaphase-arrested cells in the presence or absence of tension. f, Mean Sgo1 signal centred around all 32 borders (left) or 16 centromeres (right) (solid lines, medians; dark shading, standard errors; light shading, 95% confidence intervals).