Extended Data Fig. 2: Topography rescues T cell locomotion in the absence of adhesion.
a–c, Migration of control (n = 71) and talin-KO (n = 74) cells under 3-μm confinement. Representative of four independent experiments. In a, the left panel shows the scheme of the 3-μm confinement used in a–c. The right panel shows representative snapshots of control and talin-KO cells under 3-μm confinement at t = 60 min. Individual tracks are displayed in different colours. Scale bars, 50 μm. In b, the speed and displacement of control and talin-KO cells under 3-μm confinement are shown. Data are mean ± s.d.; ****P < 0.0001, two-sided Mann–Whitney U-test. In c, the MSD of control cells (black) and talin-KO cells (red) is shown. Data are mean ± s.e.m.; P < 0.0001, two-sided Mann–Whitney U-test. d, Related to Fig. 2a–d. Scheme of multilayered microfluidic devices used to push cells into the pillar maze or into microchannels used in e and f. e, Snapshots of video microscopy at t = 60 min of control and talin-KO cells in the pillar device. The nucleus is shown in cyan (Hoechst), Lifeact-GFP reporter in red and the bright-field image in grey. Individual cell tracks are displayed in yellow. Scale bars, 50 μm. Time is in min:s. Representative of three experiments. f, Related to Fig. 2g, h. Velocities (μm/min) of control and talin-KO cells in serrated channels of different diameters (with a conserved serration period of 6 μm). Representative of three experiments. n = 57 for control cells and n = 9 for talin-KO cells. *P = 0.0356; otherwise not significant, Kruskal–Wallis one-way ANOVA followed by post hoc Dunn’s test. Boxes extend from the 25th to the 75th percentiles, with the middle line showing the median and the whiskers representing the minimum to maximum values.
