Extended Data Fig. 3: Topography rescues dendritic cell locomotion in the absence of adhesion.
a, Top left, scheme of a mature dendritic cell migrating in a channel designed for the EDTA experiment. Mature dendritic cells migrate in a device containing channels with different serration periods, with or without 30 mM EDTA. Top right, single-cell migration speed with (+, blue) and without (-, grey) EDTA treatment. Bottom, histograms of cell speed in different serrations with (blue) or without (grey) EDTA. Representative of three independent experiments. n = 35 cells without EDTA, n = 17 cells with EDTA. Data are mean ± s.d.; **P = 0.0022, ****P < 0.0001 and ns P = 0.6363, one-way ANOVA with Kruskal–Wallis test followed by post hoc Dunn’s test. b, Top left, scheme of a mature dendritic cell migrating in a channel designed for the EDTA experiment, along a CCL19 gradient (indicated in red). Mature dendritic cells migrate towards the chemokine CCL19 into a device that contains microchannels with different serration periods, with or without 30 mM EDTA. Top right, migration speed with (+, blue) and without (-, grey) EDTA treatment. Bottom, histograms of cell speed in the different serrated channels with (blue) or without (grey) EDTA. Representative of three independent experiments. Without EDTA: n = 44 cells; with EDTA: n = 52 (6–12 μm), n = 44 (24 μm), n = 33 (smooth channels). Data are mean ± s.d.; ***P = 0.0001 and ****P < 0.0001, one-way ANOVA with Kruskal–Wallis test followed by post hoc Dunn’s test.
