Extended Data Fig. 2: Enzymatic activity of recombinant human ACAT1.
From: Structural basis for catalysis and substrate specificity of human ACAT1
a, Schematic of our fluorescence-based activity assay for ACAT1. Top, the chemical structure of CoASH; bottom, a schematic illustration of the fluorescence-based activity assay. b, Interference of detergents on the enzymatic activity of ACAT1. The proteins used for the assay were purified by SEC in the presence of 1% CHAPS or 0.02% GDN. c, Allosteric activation of ACAT1 by cholesterol. The sigmoidal plot of catalytic activity with increasing concentrations of cholesterol is consistent with the proposed allosteric activation of ACAT1 by cholesterol32. Data in b, c are mean ± s.d. of three independent experiments.
