Extended Data Fig. 3: Cryo-EM analysis of the structure of human ACAT1.

a, A representative micrograph (left) and 2D class averages (right) of cryo-samples of ACAT1 in GDN micelles. The box size for 2D averages is 310 Å. b, FSC curves for the 3D electron microscopy reconstructions of tetrameric and dimeric ACAT1. c, Local resolution map of tetrameric (top) and dimeric (bottom) ACAT1 calculated using RELION 3.0. The resolution bars on the right are labelled in Å. d, Directional FSC (dFSC) for the dimeric reconstruction. Each purple curve indicates a different direction. In total, 500 dFSC curves were generated, which were averaged and shown by the green curve (average dFSC)⁵¹. e, FSC curves of the refined model versus the summed map that it was refined against (black); of the model refined in the first of the two independent maps used for the gold-standard FSC versus that same map (red); and of the model refined in the first of the two independent maps versus the second independent map (green) for the dimeric reconstruction.