Extended Data Fig. 6: Structural comparison of ACAT1 and DltB.
From: Structural basis for catalysis and substrate specificity of human ACAT1
a, ACAT1 and DltB share an identical structural core. TM2–TM9 of ACAT1 can be superimposed onto TM3–TM10 of DltB (PDB ID: 6BUI) with an r.m.s.d. of 5.0 Å over 272 Cα atoms. Superimposition of ACAT1 onto DltB is shown in two perpendicular side views. The major conformational shifts of TM8 and TM9in ACAT1 from the corresponding segments in DltB are indicated with orange arrows. TM1 of ACAT1 and the corresponding segments TM1 and TM2 (dark grey) in DltB adopt different structures. b, Loop1 and Loop2 constitute the major cytosolic segments in both ACAT1 and DltB. The cytosolic views of the two proteins, with corresponding structural segments coloured the same, are shown here. c, There is no C tunnel in DltB as there is in ACAT1. The electrostatic surface potentials of ACAT1 and DltB are shown in the same cut-open side views. The conserved His residue is shown as magenta sticks in both structures.
