Extended Data Fig. 8: Alternative methods to normalize deuterium uptake.

a, Deuterium uptake difference between monomer (0.67 μM) and dimer (75 μM) at each time point was normalized by the length of each peptide. Peptides were categorized by the interface to which they contribute, as in Fig. 2c. *Interface peptide sets that show significantly increased uptake upon dilution when compared to peptides outside of that interface, as determined by a permutation test (see Extended Data Fig. 6). Each point shows the mean ± s.e. from three replicates. b, Permutation test to evaluate the difference in deuterium uptake at 60 min by peptides at each interface, when uptake difference per peptide is normalized by length (as described in Extended Data Fig. 6g). Orange, peptides with IF1-containing residues versus those with no IF1 residues. Yellow, IF2-containing peptides versus those with no IF2 residues. Dashed line, P = 0.05. c, d, Average deuterium uptake difference per residue (c) and uptake difference normalized by dimer uptake (d) for peptides at different time points. Orange, IF1 sites; yellow, IF2 sites. Each rectangle shows the position of the peptide in the linear sequence and its uptake (mean of three replicates).