Extended Data Fig. 6: Loss of LRRC32 and GARP expression in Foxp3⁺ T_reg cells from Enh-KO mice.

a, Scatter plot showing global differences in chromatin accessibility at called peaks using ATAC-Seq analysis of WT and Enh-KO CD4⁺ Foxp3^GFP+ T_reg cells. Red dots show significantly differentially accessible peaks (FDR < 0.05). Mean log₂ reads per million (RPM) values for each called peak (represented as points) from three independent biological replicates are shown, with samples isolated on different days. Two-tailed Wald test with Benjamini–Hochberg correction. b, Representative alignment of gene expression (top) and chromatin accessibility (bottom) within the indicated cell types sorted by FACS from WT and Enh-KO Foxp3^eGFP reporter mice. Expected loss of ATAC-seq reads mapping to the deleted region (Enh; highlighted in grey) in Enh-KO cells is observed. Data representative of three independent biological replicates with samples isolated on different days. c, Representative flow cytometry analysis of GARP and Foxp3 expression by CD4⁺ T cells of mice of the indicated genotypes from mesenteric lymph nodes. d, GARP expression from mice of indicated genotypes in spleen (n = 5 per genotype), thymus (n = 6 and 5 for WT and Enh-KO groups), and mesenteric lymph node (n = 3 and 4 for WT and Enh-KO groups). Data are representative of three and two independent experiments (c, d). Unpaired two-tailed Student’s t test (d). Data are mean ± s.e.m.

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