Extended Data Fig. 6: Two distinct waves of yolk sac-derived macrophages contribute to TRM populations in human embryos.
From: Deciphering human macrophage development at single-cell resolution
a, The area coloured lavender on the UMAP visualization highlights the myeloid groups selected for re-analysis (n = 8 biologically independent embryo samples and 1,231 cells). b, UMAP visualization of all the haematopoietic cells with re-clustered myeloid and macrophage clusters (n = 782 myeloid cells) mapped on. c, Heat map showing scaled expression of the top five DEGs for each re-clustered myeloid and macrophage population. DEGs were detected using FindAllMarkers function in Seurat (one-sided Wilcoxon rank-sum test, with P value adjusted for multiple testing using Bonferroni correction), and genes with fold change >1.5 and adjusted P < 0.05 were selected. d, UMAP visualization of myeloid cells with stage (top) and site (bottom) information mapped on. e, Heat map showing scaled expression of curated TRM signature genes from a previous mouse study24 in the re-clustered macrophage populations (n = 450 cells: 7 Liver_Mac cells, 51 Blood_Mac cells, 71 Lung_Mac cells, 46 Skin_Mac cells, 61 YS_Mac1 cells, 29 YS_Mac2 cells, 73 Head_Mac1 cells, 38 Head_Mac2 cells, 41 Head_Mac3 cells, and 33 Head_Mac4 cells).
