Extended Data Fig. 8: Chondral development in the tail region of skin organoids.
From: Hair-bearing human skin generated entirely from pluripotent stem cells
a, b, Violin and t-SNE plots showing normalized expression of chondral marker genes within cluster 7 of the day-48 WA25 skin organoid dataset (Extended Data Fig. 6, Supplementary Data 7). LECT1 is also known as CNMD. The data represent cells pooled from six day-48 skin organoids from one experiment. c, Haematoxylin-stained section of a day-140 skin organoid showing hyaline cartilage that has formed within skin organoid-associated mesenchymal tissue. Three independent haematoxylin stainings were performed on six skin organoids from three different experiments. d, TEM image of two representative chondrocytes located within hyaline cartilage tissue (dashed box in c). TEM was performed once on two different skin organoids. e, f, Immunostaining of day-140 organoid samples for aggrecan (ACAN) and collagen type II alpha 1 (COL2A1) highlights cartilage development. Images represent one of three independent IHC stainings on six skin organoids produced from three separate experiments. Scale bars, 100 μm (c–f). This figure corresponds with the data in Figs. 2, 3.
