Extended Data Fig. 1: Analysis of plasma binding to cell surface expressed trimeric spike protein.
From: Human neutralizing antibodies elicited by SARS-CoV-2 infection
HEK 293T cells transfected with expression plasmid encoding the full length spike of SARS-CoV-2, SARS-CoV or MERS-CoV were incubated with 1:100 dilutions of plasma from the study subjects. The cells were then stained with PE labelled anti-human IgG Fc secondary antibody and analysed by FACS. Positive control antibodies include S230 and m396 targeting the RBD of SARS-CoV spike, and Mab-GD33 targeting the RBD of MERS-CoV spike. VRC01 is negative control antibody targeting HIV-1 envelope glycoprotein. Results presented are representatives of two independent experiments.
