Extended Data Fig. 7: Constructs for expressing the bai operon and portions thereof in C. sporogenes.
From: A metabolic pathway for bile acid dehydroxylation by the gut microbiome
Each of the plasmids has replication origins (origin and repH) for E. coli and Clostridium, the traJ gene to enable conjugal plasmid transfer, and an antibiotic-resistance gene (catP, aad9 or ermB). The bai genes were introduced into these plasmids under the control of the fdx or spoIIE promoter. For the genetic analysis of baiCD and baiH function, pMTL83153-based plasmids were used.
