Extended Data Fig. 9: The effects of a PAM on signalling of the site-1 and -2 mutants of GABAB.
From: Structural basis of the activation of a metabotropic GABA receptor
a, IP1 production induced by 10 μM GS39783 in intact cells expressing the indicated site-1 GB2 mutants co-expressed with the wild-type GB1. Data are normalized by the GABA response and shown as mean ± s.d. of 7 (10 for wild type) biologically independent experiments. Data are analysed using one-way ANOVA with Dunnett’s multiple comparison test to determine significance (compared with wild type), with P = 0.9998 for GB2(R556A), 0.9999 for GB2(H647A), 0.9907 for GB2(L657A), 0.9995 for GB1(F711A), 0.9997 for GB2(R714A), 0.9996 for GB2(Q720A), and 0.2807 for GB2(SFR-AAA). b–d, IP1 production induced by GABA (blue) or GS39783 (red) in intact cells expressing the wild-type receptor (b) or the indicated mutant of the site 1 (c) or site 2 (d). Data are normalized by the GABA response and shown as mean ± s.e.m. of four biologically independent experiments.
