Extended Data Fig. 4: Cryo-EM data processing for SKF97541- and GS39783-bound GABAB.
From: Structural basis of the activation of a metabotropic GABA receptor
a, Single-particle cryo-EM data processing scheme using cryoSPARC. b, Micrograph showing picked particles. c, Representative 2D class averages show distinct secondary structure features. d, Particle angular distribution of the final cryo-EM reconstruction. e, Structural model shown in cartoon representation and coloured by the B-factors. f, Density map coloured by local resolution, showing higher resolution at the interface between GB1 and GB2. g, The heterodimer interface includes both LB1 and LB2, as well as TM6, with a total interface area of 1,930 Å2. Residues involved in dimerization contacts are coloured in purple.
